Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: “Shepherd’s crook” neurons drive and synchronize the enhancing and suppressive mechanisms of the midbrain stimulus selection network
doi: 10.1073/pnas.1804517115
Figure Lengend Snippet: In vitro injections of fluorescent tracers in TeO and the isthmi. (A) Photomicrograph of a chicken midbrain horizontal slice showing an anterograde tracing experiment with an injection site of B-TMR in the intermediate layers of TeO. A, Inset represents the approximate section plane and position of the isthmic nuclei. (B) Labeled Shc axons leave regularly spaced collaterals (arrows) and continue their course outside the Imc nucleus (asterisks). (C) Retrograde tracing experiment showing the injection sites of B-TMR in Ipc and fluorescein-conjugated BDA (BDA-F) in Imc. (D) Merged channels showing the double-labeled Shcs. (E) Red channel, B-TMR–labeled Shcs in tectal layer 10 from the injections in C. (F) Green channel, BDA-F–labeled Shcs. [Scale bars, 500 µm (A and C); 100 µm (B and D–F).]
Article Snippet: Injections of fluorescent tracers were performed by using a nanoinjector (WPI) and borosilicate pipettes, 20-μm tip, filled with a 10% dilution in phosphate buffer of either tetramethylrhodamine-conjugated biocytin (B-TMR) (Invitrogen) or fluorescein-conjugated BDA (3k; Invitrogen).
Techniques: In Vitro, Anterograde Tracing, Injection, Labeling, Retrograde Tracing